Journal: Cell reports

Article Title: Dentate Gyrus and CA3 GABAergic Interneurons Bidirectionally Modulate Signatures of Internal and External Drive to CA1

doi: 10.1016/j.celrep.2021.110159

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 1 μL of 4.6 × 10 12 vg/mL AAV5-hSyn-DIO-hM4D(Gi)-mCherry (UNC Viral Vector Core; ( Krashes et al., 2011 )) or 7 × 10 12 vg/mL AAV5-hSyn-DIO-mCherry (Addgene) was injected at 2.1 mm below the surface of the brain ( Andrews-Zwilling et al., 2012 ; Stefanelli et al., 2016 ) at an infusion rate of 100nL/min.

Techniques: Plasmid Preparation, Recombinant, Blocking Assay, Software

Journal: Cell reports

Article Title: Dentate Gyrus and CA3 GABAergic Interneurons Bidirectionally Modulate Signatures of Internal and External Drive to CA1

doi: 10.1016/j.celrep.2021.110159

Figure Lengend Snippet: (A) Experimental strategy. Mice were bilaterally injected with AAV5-hSyn-DIO-hM4D(Gi)-mCherry or with AAV5-hSyn-DIO-mCherry into the DG hilus, then implanted with a 32-site 4-shank silicon electrode array into the right dorsal hippocampus. LFP activity was recorded from all hippocampal subregions over 6 daily 1 hour sessions, alternating CNO and vehicle treatment.

Article Snippet: 1 μL of 4.6 × 10 12 vg/mL AAV5-hSyn-DIO-hM4D(Gi)-mCherry (UNC Viral Vector Core; ( Krashes et al., 2011 )) or 7 × 10 12 vg/mL AAV5-hSyn-DIO-mCherry (Addgene) was injected at 2.1 mm below the surface of the brain ( Andrews-Zwilling et al., 2012 ; Stefanelli et al., 2016 ) at an infusion rate of 100nL/min.

Techniques: Injection, Activity Assay

Journal: Cell reports

Article Title: Dentate Gyrus and CA3 GABAergic Interneurons Bidirectionally Modulate Signatures of Internal and External Drive to CA1

doi: 10.1016/j.celrep.2021.110159

Figure Lengend Snippet: (A) Experimental strategy. Mice were bilaterally injected with AAV5-hSyn-DIO-hM4D(Gi)-mCherry into the DG hilus, then implanted with a 32-site single-shank silicon electrode array into the right dorsal hippocampus. LFP activity was recorded during sleep and awake rest over 6 daily 1 hour home cage session, alternating CNO and vehicle treatment.

Article Snippet: 1 μL of 4.6 × 10 12 vg/mL AAV5-hSyn-DIO-hM4D(Gi)-mCherry (UNC Viral Vector Core; ( Krashes et al., 2011 )) or 7 × 10 12 vg/mL AAV5-hSyn-DIO-mCherry (Addgene) was injected at 2.1 mm below the surface of the brain ( Andrews-Zwilling et al., 2012 ; Stefanelli et al., 2016 ) at an infusion rate of 100nL/min.

Techniques: Injection, Activity Assay

Journal: Cell reports

Article Title: Dentate Gyrus and CA3 GABAergic Interneurons Bidirectionally Modulate Signatures of Internal and External Drive to CA1

doi: 10.1016/j.celrep.2021.110159

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 1 μL of 4.6 × 10 12 vg/mL AAV5-hSyn-DIO-hM4D(Gi)-mCherry (UNC Viral Vector Core; ( Krashes et al., 2011 )) or 7 × 10 12 vg/mL AAV5-hSyn-DIO-mCherry (Addgene) was injected at 2.1 mm below the surface of the brain ( Andrews-Zwilling et al., 2012 ; Stefanelli et al., 2016 ) at an infusion rate of 100nL/min.

Techniques: Plasmid Preparation, Recombinant, Blocking Assay, Software

Journal: Neuron

Article Title: Identification of Spinal Neurons Contributing to the Dorsal Column Projection Mediating Fine Touch and Corrective Motor Movements

doi: 10.1016/j.neuron.2019.08.029

Figure Lengend Snippet: (A) Experimental strategy for monosynaptic retrograde tracing of Zic2cre spinal neurons. CAG promoter; loxP, recognition sites for Cre recombinase. An EnvA-pseudotyped G-deleted eGFP virus (ΔG-GFP) was injected intraspinally at L2/L3 or C2 levels in P7 pups (right panel).

Article Snippet: EnvA ΔG rabies-eGFP , Viral Vector Core, Salk Institute ( Osakada et al., 2011 ) , Addgene#32635; RRID:Addgene_32635.

Techniques: Retrograde Tracing, Virus, Injection